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Open Access Letter to the Editor

A novel monoclonal antibody of human stem cell factor inhibits umbilical cord blood stem cell ex vivo expansion

Jie Fan1, Xinxin Ding1 and Yongping Jiang12*

Author Affiliations

1 Biopharmaceutical R&D Center, Chinese Academy of Medical Sciences & Peking Union Medical College, Suzhou 215126, China

2 Biopharmagen Corp, Suzhou 215126, China

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Journal of Hematology & Oncology 2012, 5:73  doi:10.1186/1756-8722-5-73

Published: 10 December 2012

Abstract

Stem cell factor (SCF) activates hematopoietic stem cell (HSC) self-renewal and is being used to stimulate the ex vivo expansion of HSCs. The mechanism by which SCF supports expansion of HSCs remains poorly understood. In cord blood ex vivo expansion assays, a newly produced anti-SCF monoclonal antibody (clone 23C8) was found to significantly inhibit the expansion of CD34+ cells. This antibody appears to bind directly to a part of SCF that is critical for biological activity toward expansion of CD34+ cells, which is located in the first 104 amino acids from the NH2-terminus.

Keywords:
Hematopoietic stem cell; Stem cell factor; Umbilical cord blood; Monoclonal antibody