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SU11652 Inhibits tyrosine kinase activity of FLT3 and growth of MV-4-11 cells

Yao Guo12, Yun Chen2, Xuesong Xu13, Xueqi Fu1* and Zhizhuang Joe Zhao12*

Author Affiliations

1 Edmond H. Fischer Signal Transduction Laboratory, College of Life Sciences, Jilin University, Changchun, China

2 Department of Pathology, University of Oklahoma Health Sciences Center, Oklahoma City, Oklahoma, 73104, USA

3 Clinical Laboratory, China Japan Union Hospital, Jilin University, Changchun, China

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Journal of Hematology & Oncology 2012, 5:72  doi:10.1186/1756-8722-5-72


The electronic version of this article is the complete one and can be found online at: http://www.jhoonline.org/content/5/1/72


Received:24 October 2012
Accepted:26 November 2012
Published:6 December 2012

© 2012 Guo et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background

FLT3-ITD and FLT3-TKD mutations are frequently found in acute myeloid leukemia (AML). This makes tyrosine kinase FLT3 a highly attractive target for therapeutic drug development. However, effective drugs have not yet emerged. This study is intended to identify and to characterize new FLT3 inhibitors.

Methods

By using the protein substrate GST-FLT3S to analyze kinase activity of recombinant proteins carrying the catalytic domain of wild type and mutant forms of FLT3, we screened a chemical library containing 80 known protein kinase inhibitors. We identified SU11652 as a potent FLT3 inhibitor and further employed FLT3-ITD-positive MV- 4–11 cells to study its effects on cell growth, apoptosis, cell cycles, and cell signaling.

Results

SU11652 strongly inhibited the activity of wild type, D835Y, and D835H mutant forms of FLT3 with IC50 values of 1.5, 16, and 32 nM, respectively. It effectively blocked the growth of FLT3-ITD -positive MV-4-11 cells at nanomolar concentrations but exhibited much less effects on several other cells which do not carry mutations of FLT3. SU11652 inhibited growth of MV-4-11 cells by inducing apoptosis, causing cell cycle arrest, and blocking activation of the ERK, Akt, and STAT signaling pathways.

Conclusion

SU11652 is a potent FLT3 inhibitor which selectively targets FLT3-ITD-positive cells. It should serve as a good candidate for development of therapeutic drugs to treat AML.

Keywords:
Tyrosine kinase; FLT3; Inhibitor screening; SU11652; Acute myeloid leukemia

Background

Acute myeloid leukemia (AML) is the most common malignant myeloid disorder in adults and is featured by abnormal differentiation and proliferation of hematopoietic progenitor cells of the myeloid lineage [1]. The median age of AML patients at diagnosis is 67 years, and it is estimated that 13,780 (7,350 men and 6,430 women) will be diagnosed with and 10,200 will die of the disease in 2012 in USA [2]. Currently, there is no effective cure for the disease. With standard chemotherapy, the 3-year event-free survival rate of patients with favorable cytogenetics is 60%; however, the median survival of patients with unfavorable cytogenetics is only 6 months [1,3]. Therefore, novel therapies with higher efficacy and fewer side effects are badly needed. With the increased understanding of the pathogenesis of AML, several molecular lesions have been identified, which allows development of targeted drugs [4]. Among them, FMS-like tyrosine kinase 3 (FLT3) is one of the most attractive targets, and related drug therapies are currently under development [5,6].

FLT3 is a receptor tyrosine kinase mainly expressed in early myeloid and lymphoid progenitors, and it plays a role in the development of hematopoietic progenitor cells [7]. Mutations of FLT3 have been found in about 30% of AML patients [8]. Among these mutations, the most common type is FLT3-ITD caused by internal tandem duplication (ITD) in the juxtamembrane domain, and the less common FLT3-TKD type involves point mutations in the tyrosine kinase domain (TKD). These mutations lead to constitutive activation of FLT3 and result in uncontrolled proliferation of leukemia cells [9], and they are strongly associated with poor prognosis in AML patients [10]. Since mutations found in AML cause activation of FLT3, FLT3 has become an obvious target for anticancer drugs. Up to date, several small molecule inhibitors and monoclonal antibodies against FLT3 have been developed [11-14]. Clinical trials have shown the efficacy of some FLT3 inhibitors in treatment of AML, but the responses are incomplete and often limited by the acquired resistance during the treatment [15-17]. For off-label use, sorafenib, which was initially identified as an inhibitor of VEGFR/PDGFR tyrosine kinases and Raf kinases, is the only FDA-approved anticancer drug targeting FLT3 [18]. Needless to say, identification of novel FLT3 inhibitors with higher potency is required.

In this study, by screening a protein kinase inhibitor library using a protein substrate we recently developed [19], we identified SU11652 as a potent FLT3 inhibitor. We further performed cell-based assays to demonstrate its inhibitory selectivity and potency for FLT3-ITD-positive cells and the cellular and molecular mechanism underlying its action. We believe that SU11652 should serve as a good candidate for development of targeted drugs to treat AML.

Results and discussion

SU11652 is a potent FLT3 inhibitor

In our previous studies, we developed a system to analyze kinase activity of FLT3 by using a protein substrate [19]. We now employed the assays to screen chemical libraries for identification of FLT3 inhibitors. The library that we chose for this study is the InhibitorSelect™ 96-Well Protein Kinase Inhibitor Library I from Calbiochem. The library consists of 80 cell-permeable protein kinase inhibitors. The compounds were supplied at a concentration of 10 mM in dimethyl sulfoxide (DMSO). Our initial screening was performed with 3 μM and then 0.3 μM of each inhibitor. This led to identification of several compounds that exhibited strong inhibitory effects on FLT3 and its FLT3D835Y and FLT3 D835H mutant forms (data not shown). One of the compounds, SU11652, was further characterized. Figure 1 shows the dose responses. SU11652 inhibited the kinase activity of wild type FLT3 with an IC50 value of ~1.5 nM. It displayed relatively lower potency toward the FLT3D835Y and FLT3D835H mutants with IC50 values of 16 and 32 nM, respectively. SU11652 is a sunitinib-like oxindole inhibitor and has been found as an inhibitor of PDGFRβ, VEGFR2, FGFR1, Flk-1, and cKit with IC50 or Ki values of 3–500 nM [20,21]. Our study now suggests that it inhibits FLT3 with even higher potency. It is interesting to note that the D835Y and D835H mutant forms of FLT3 are less sensitive to SU11652 than the wild type FLT3. This is reminiscent of data obtained with two other known FLT3 inhibitors, namely, sorafenib and AC220 (quizartinib) [15]. On the other hand, FLT3-ITD mutants contain the wild type kinase domain and should be highly sensitive to inhibition by SU11652. Therefore, in the clinical applications, SU11652 would be more suitable for patients with FLT3-ITD mutations than those with FLT3-TDK mutations.

thumbnailFigure 1. SU11652 is a potent FLT3 inhibitor. A. Tyrosine kinase activity of recombinant FLT3, FLT3D835H, and FLT3 D835Y was analyzed with GST-FLT3S as the substrate in the presence of various concentrations of SU11652. Tyrosine phosphorylation of GST-FLT3S was detected by using anti-phosphotyrosine antibody PY20, and its protein level, by Coomassie blue staining. B. The relative kinase activity was calculated based on the density of the western blot bands normalized to the control group. Error bars denote standard deviation (n = 3).

SU11652 inhibits the growth of FLT3-ITD-positive cells

We further employed cell-based assays to verify the inhibitory effects of SU11652 on FLT3. For this purpose, the MV-4-11 cell line was employed. The cells were derived from biphenotypic B myelomonocytic leukemia and carry a FLT3-ITD mutation [22]. As expected, MTT assays revealed that MV-4-11 cells were highly sensitive to SU11652 with an IC50 value of ~5 nM (Figure 2A). In contrast, HL-60 acute promyelocytic leukemia, Jurkat acute T cell leukemia cells, and Karpas 299 anaplastic large cell lymphoma cells were hardly affected by the inhibitor at 500 nM. These cells do not carry FLT3 mutations. The data demonstrate that SU11652 specifically targets cells containing FLT3-ITD. It should be noted that Karpas 299 cells contain a mutation of tyrosine kinase Alk and a p53 mutation [23,24]. Apparently, SU11652 is selective for tyrosine kinases mutated in cancer cells. The inhibitory effects of SU11652 on the growth of MV-4-11 cells were also demonstrated by Wright-Giemsa staining of cells fixed to glass slides by cytospin. As shown in Figure 2B, in comparison with the non-treated MV-4-11 cells, cells treated with 100 nM SU11652 were sparser and smaller, showing no mitotic cells but rather condensed nuclei and cell debris. As a control, HL-60 cells behaved normally showing regular morphology and many mitotic cells in the presence of 100 nM SU11652.

thumbnailFigure 2. SU11652 effectively inhibits the growth of MV-4-11 cells. A. MV-4-11, HL-60, Karpas 299, and Jurkat cells were cultured in the presence of various concentrations of SU11652 for 48 hours. Cell viability was assessed by MTT assays. Error bars denote standard deviation (n = 3). B. Wright-Giemsa staining of MV-4-11 and HL-60 cells treated with 0 or 100 nM SU11652 for 48 h. Black arrows point to mitotic cells.

SU11652 induces apoptosis and cell cycle arrest in MV-4-11 cells

To reveal further how SU11652 inhibits the growth of MV-4–11 cells, we conducted apoptosis assays and cell cycle analyses. Apoptosis was demonstrated by staining with Annexin V and propidium iodide. As shown in Figure 3, the percentage of Annexin V-positive and propidium iodide-negative cells was increased following SU11652 treatment, reaching 17.7% at 100 nM, indicating induction of apoptotic cell death. The effects of SU11652 on the cell cycle were even more prominent. As shown in Figure 4, treatment of MV-4-11 cells with 10 nM SU11652 reduced S phase cells from 24.7% to 7.6%, accompanied by comparable reduction in the percentages of G2 phase cells. Together, the data indicate that SU11652 induces both apoptosis and cell cycle arrest of MV-4-11 cells.

thumbnailFigure 3. SU11652 induces apoptosis of MV-4-11 cells. MV-4-11 cells were incubated with 0, 10 and 100 nM SU11652 for 24 hours. Cells were stained with Cy5-labeled annexin V and propidium iodide, followed by analyses with a flow cytometry. Percentages of annexin V-positive and propidium iodide-negative cells, that is, apoptotic cells, are indicated.

thumbnailFigure 4. SU11652 induces cell cycle arrest of MV-4-11 cells. MV-4-11 cells were incubated with 0, 10, and 100 nM SU11652 for 24 hours. Cells were fixed with ethanol and stained with propidium iodide before flow cytometric analysis. Percentages of cells in G1, S, and G2 phases were calculated by using the ModFit software.

SU11652 inhibits FLT3 downstream signaling in MV-4-11 cells

As a growth factor receptor, FLT3 turns on various signaling pathways to regulate cell growth and proliferation [9]. These include the Ras/MAPK, PI3K/Akt, and JAK/STAT pathways. Mutations of FLT3 are known to cause constitutive activation of FLT3 kinase activity and downstream signaling components [9]. To see if the inhibition of FLT3 kinase activity by SU11652 also blocks its downstream signal transduction events, we used phospho-specific antibodies to detect activation of ERK1/2, Akt, and STAT5 in MV-4-11 cells. Figure 5 shows a dose-dependent reduction in the phosphorylation levels of FLT3, ERK1/2, Akt, and STAT5 in MV-4-11 cells treated with SU11652. This indicates that the activation of these downstream signaling proteins in MV-4–11 is blocked by SU11652, thereby providing a molecular mechanism for the inhibition of cell growth by the inhibitor.

thumbnailFigure 5. SU11652 blocks activation of signal transduction pathways downstream of FLT3. MV-4-11 cells were treated with the indicated concentrations of SU11652 for 24 hours. Cell extracts were subjected to western blot analyses with antibodies against phosphorylated forms of FLT3 (pY591), ERK1/2 (pT202/pY204), Akt (pS473), and STAT5 (pY694). Equal protein loading was demonstrated by blotting with house-keeping gene product GAPDH.

Conclusions

By screening a chemical library with an effective FLT3 kinase assay system, we have identified SU11652 as a potent inhibitor of FLT3 with an IC50 value of 1.5 nM. More importantly, our in vitro cell-based assays demonstrated that SU11652 selectively inhibited the growth of FLT3-ITD-positive MV-4-11cells with equivalent potency. Furthermore, we showed that SU11652 induced apoptosis, caused cell cycle arrest, and blocked FLT3 downstream signaling transduction. FLT3 is an obvious target for therapeutic drugs to AML, but no effective drug has emerged. Our study provides a new candidate. Considering the potency and selectivity of SU11652 according to biochemical and cell-based assays, further preclinical study with animal models and clinical studies with FLT3-ITD -positive AML patients appears to be well warranted.

Methods

Materials

InhibitorSelect Protein Kinase Library I containing 80 protein kinase inhibitors including SU11652 was purchased from Calbiochem (CA, USA). Monoclonal anti-phosphotyrosine antibody PY20 was from BD Biosciences (CA, USA), while antibodies against pFLT3 (pY591), pERK1/2(pT202/pY204), pAKT(pS473), and pSTAT5(pY694) were from Cell Signaling Technology (MA, USA). MV-4–11, HL-60, and Jurkat cell lines were obtained from ATCC (VA, USA). Karpas 299 cells were kindly provided by Yi Zhao (University of Southern California, [23]). MV-4-11 cells were cultured in Iscove’s Modified Dulbecco’s Medium containing 10% fetal bovine serum, and the rest of cells were maintained in RPMI medium supplemented with 10% fetal bovine serum.

FLT3 kinase activity assays and inhibitor screening

Protein kinase activity assays and inhibitor screening were performed as previously described [19,25]. The FLT3 substrate GST fusion protein GST-FLT3S was purified from E. coli cells by using a glutathione-Sepharose column, and recombinant proteins containing the catalytic domain of wild type FLT3 and its D835H and D835Y mutant forms were isolated from recombinant baculovirus-infected Sf9 insect cells by using the NTA-Ni resin [19]. Phosphorylation of GST-FLT3S by isolated FLT3 tyrosine kinases was carried out in a reaction buffer containing 25 mM Tris–HCl (pH 7.5), 10 mM MgCl2, 0.2 mM adenosine 5-triphosphate, and 2 mM dithiothreitol in the presence of various concentrations of protein kinase inhibitors. The level of GST-FLT3S tyrosine phosphorylation was determined by immunoblotting with anti-phosphotyrosine antibody PY20 followed by horseradish peroxidase-conjugated secondary antibody. Detection and quantification of enhanced chemiluminescence signals were done by using FluorChem SP imaging system from Alpha Innotech [26].

Cell viability assays

MV-4-11, HL-60, Karpas 299, and Jurkat cells were incubated with various concentrations of SU11652 for 48 hours. To measure the viability of cells, 0.5 mg/ml MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was added into the medium. After incubation at 37°C for 3 hours, the medium was removed by centrifugation and the precipitated dye was dissolved in 1 ml isopropanol containing 0.04 M HCl. Absorbance at 570 nm was then measured with a spectrophotometer.

Apoptosis and cell cycle analyses

For apoptosis analysis, the cells were stained with Annexin V-Cy5 and propidium iodide (Biovision, CA, USA). To assess cell cycle arrest, the cells were fixed with ethanol overnight and then stained with propidium iodide in the presence of RNAse. Flow cytometric assays were performed by using a FACSCalibur flow cytometer (BD Biosciences) at the Flow and Image Cytometry Laboratory of University of Oklahoma Health Sciences Center.

Cell signaling assays

Cells treated with SU11652 or the control solvent were extracted with a whole-cell extraction buffer containing 25 mM β-glycerophosphate (pH 7.3), 5 mM EDTA, 2 mM EGTA, 5 mM β-mercaptoethanol, 1% Triton X-100, 0.1 M NaCl, 1 mM sodium vanadate, and a protease inhibitor cocktail (Roche Applied Science, Indianapolis, IN, USA). Cell lysates were cleared by centrifugation in a microfuge at 13,000 g, and clear cell extracts containing equal amounts of total proteins were separated on SDS gels for western blot analyses with antibodies against pFLT3, pERK, pAKT, and pSTAT5.

Abbreviations

AML: Acute myeloid leukemia; GST: Glutathione S-transferase.

Competing interests

The authors declare no conflict of interests.

Authors’ contributions

GY and YC performed the research experiments; XX designed the research; XF and ZJZ designed and supervised the research. All authors wrote and approved the manuscript.

Acknowledgements

This work was supported by grants HL079441 and HL094591 from the National Institutes of Health, and a grant from Oklahoma Center for the Advancement of Science & Technology (to ZJ Zhao).

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