Table 2 |
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|
Adoptive transfer of CD4 T cells populations into B16-OVA tumor-bearing mice. |
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|
Percent Foxp3EGFP Positive Cells Received From Spleen and Tumor |
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|
T Cell Subsets Adoptively Transferred |
Spleen day 5 |
Tumor day 5 |
Spleen day 10 |
Tumor day 10 |
Spleen day 15 |
Tumor day 15 |
|
|
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|
Bone marrow control (0%) |
0.0, 0.0 |
0.0, 0.0 |
0.0, 0.0 |
|||
|
C57BL/6 CD4 (0%) |
0.0, 0.0 |
0.3, 0.5 |
0.0,0.0 |
0.0, 0.0 |
||
|
C57BL/6 nTreg CD4 (100%) |
1.4, 1.9 |
11.7, 15.2 |
14.8, 20.8 |
|||
|
C57BL/6 iTreg CD4 (26%) |
0.4, 0.3 |
4.5, 3.8 |
0.4, 2.0 |
|||
|
OTII CD4 (0%) |
0.0, 0.1 |
0.0, 0.0 |
0.0, 0.0 |
0.0, 0.0 |
||
|
OTII iTreg CD4 (10.4%) |
0.2, 0.0 |
0.0, 0.0 |
0.9, 1.4 |
0.1, 0.1 |
0.3, 0.4 |
|
|
SPLEEN day 7 |
TUMOR day 7 |
SPLEEN day 14 |
TUMOR day 14 |
SPLEEN day 25 |
TUMOR day 25 |
|
|
Pmel CD8 (<1%) |
0.0, 0.0 |
0.1, 0.1 |
0.0, 0.0 |
0.1, 0.1 |
0.0, 0.0 |
0.0,0.0 |
|
|
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|
C57BL/6 (CD45.2) mice bearing (five-day) B16-OVA tumors were irradiated with 900 cGy. The following day, all mice received bone marrow, from syngenic CD45.1 mice. Several different populations of cells were adoptively transferred into B16-OVA tumor-bearing mice. These cells were activated for 72 hours with αCD3/αCD28/IL-2, and in some cases flow-purified to yield a homogenous population of Foxp3EGFP-positive or negative cells. These cell populations included: (1) 105 C57BL/6 CD4 T cells (0% Foxp3EGFP); (2) 105 C57BL/6 CD4 nTreg (100% Foxp3EGFP); (3) 105 C57BL/6 CD4 TGFβ-induced iTreg (flow purified Foxp3EGFP-negative CD4 T cells, activated in the presence of TGFβ for 72 hours to yield ~26% Foxp3EGFP iTreg); (4) 105 OTII CD4 T cells (0% Foxp3EGFP); (5) 105 OTII CD4 iTreg (10.4% Foxp3EGFP); and 106 Pmel CD8 (<1% Foxp3EGFP). When sufficient numbers of TIL could be isolated for flow (at least 103), these were also surface phenotyped. |
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|
Quatromoni et al. Journal of Hematology & Oncology 2011 4:48 doi:10.1186/1756-8722-4-48 |
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