Figure 3.

Expression of Foxp3^EGFP in OTII transgenic mice. The relative percentages of Foxp3^EGFP expression were comparable in splenocytes (SPL), lymph node lymphocytes (LN), and tumor-infiltrating lymphocytes (TIL) from control or B16-OVA-tumor-bearing OTII Foxp3^EGFP mice. B16-OVA tumors were allowed to grow to 7-10 mm in diameter at which point they either did (closed square) or did not (open triangle) receive daily intraperitoneal injections of IL-2 for three days prior to analysis on the following day. In this cohort, mice did not all receive the same dose of IL2: the non-tumor bearing mouse received 250,000 units IL-2 per injection while mice in the tumor-bearing group received either 100 (one mouse), 250 (three mice), or 500 (one mouse) thousand units per injection.