Research
T cell receptor transgenic lymphocytes infiltrating murine tumors are not induced to express foxp3
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* Corresponding author: James S Economou jeconomou@mednet.ucla.edu
1 Departments of Surgery, University of California, Los Angeles, 10833 Le Conte Avenue Room 54-140 CHS Los Angeles, CA 90095 USA
2 Radiation Oncology, University of California, 10833 Le Conte Avenue, Room B3-109 CHS Los Angeles, CA 90095 USA
3 Pediatrics, University of California, 805 Tiverton, Room 12-430 Marion Davies Children's Center, Los Angeles, CA 90095 USA
4 Microbiology, Immunology and Molecular Genetics+, University of California, Los Angeles, 10833 Le Conte Avenue, Room 54-140 CHS, Los Angeles, CA 90095 USA
5 Molecular and Medical Pharmacology, University of California, 10833 Le Conte Ave, 54-140 CHS, Los Angeles, CA 90095 USA
Journal of Hematology & Oncology 2011, 4:48 doi:10.1186/1756-8722-4-48
Published: 23 November 2011Additional files
Additional file 1:
Gating Strategies for Treg populations isolated from mice. This file shows representative examples of gating for CD8 and CD4 Foxp3EGFP cells from the spleens and tumors from different transgenic mice. These mice have been well described in previous publications where gating dot plates have been illustrated (see ref #26 which describes the generation of these mice by our coauthor T. Chatila). The numbers of infiltrating transgenic T cells varies with the size of the tumor and time after infusion. See figures 1, 2, 3, Table 2.
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Additional file 2:
Gating Strategies for Treg populations generated in vitro. This file shows representative examples of gating for CD8 and CD4 Foxp3EGFP cells generated in culture by different activated cultures. See Table 1.
Format: PDF Size: 162KB Download file
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