(A) Allogeneic mixed lymphocyte reaction of normal mature Mo-DCs (-c-); immature Mo-DCs (-r-); THP-1 cells alone (-▲-); THP-1 cells with GL-PS (-○-); THP-1 DCs (-▪-) and GL-PS THP-1 DCs (-●-) with CD3+ T cells in the ratio of 1:10 and 1:100.The optical densities of incorporated BrdU from DC:T co-cultures were normalized with that from T cells alone. The results represented one experimental result of three independent experiments. The results represented the mean ± SD of three independent experiments. *p < 0.05 versus that of THP-1 cells alone. (B) CD4/CD8 ratio of the co-cultured T cells. The co-cultured T cells so harvested were stained with CD4 and CD8 antibody and analyzed with flow cytometry. The results represented the mean ± SD of two independent experiments. **p < 0.01 versus that of T cells alone.
Chan et al. Journal of Hematology & Oncology 2008 1:9 doi:10.1186/1756-8722-1-9