Figure 6.

PHI causes disruption of mitochondrial membrane potential in RPMI8226 myeloma cells. The myeloma cells were treated with 5 μM and 10 μM of PHI, respectively for 48 hours. The cells were then stained with JC-1 dye. The mitochondrial membrane potential was measured by flowcytometry as described in the Material and Methods. The shift-down of fluorescence from Red to Green indicates the collapse of mitochondrial membrane potential. CCCP was used as a positive control for the disruption of mitochondrial membrane potential. The percent of cells with the disruption of mitochondrial membrane potential was indicated.