Ras inhibition does not affect myeloid proliferation and differentiation in vitro. A). Total colony counts of sorted GFP bone marrow cells from N17 Ras and control GFP mice. The same number of cells was plated in methylcellulose media in the presence of IL-3, IL-6 and SCF. Colonies were counted 10 days after plating. B) Colonies derived from GFP sorted cells from N17 Ras and control GFP mice were morphologically characterized 10 days after plating. Four different kinds of CFU-GM colonies: Type a (I), b (II), c (III), and d (IV) were identified for both N17 Ras and GFP control mice. C) Colonies were quantified as the percentage of each of the types present for both groups. Results are shown as the mean ± standard deviation of three independent experiments.
Baum and Ren Journal of Hematology & Oncology 2008 1:5 doi:10.1186/1756-8722-1-5