Figure 5.

Alemtuzumab long-term efforts on CD34⁺ cells secondary culture. CD34⁺ cells were isolated from day 35 cultures and put into secondary culture with the original cytokine combination (50 ug/ml SCF, TPO and Flt-3 Ligand) and 10% human serum, with or without 10 μg/ml alemtuzumab (control group). After a further 7 to 14 days of culture, cells were harvested to analyze CD34 and CD52 expression profiles. A: Expression profiles of CD34 and CD52 at Day 7 and Day 14 of secondary cultures. B: Absolute expansion fold of CD34+ cells of secondary cultures. The absolute expansion fold is calculated by the absolute CD34+ cells number as compare to secondary culture initiate CD34+ numbers. The results are from three separate experiments.