Figure 1.

Detection of NPM1 mutations and FLT3-ITD using high resolution melting analysis. (A) The melt curve of NPM1 exon 12 and (B) The difference plot of NPM1 exon 12. Six patient samples are shown in comparison to five normal controls. Four patients (#6, #12, #14 and #38) are NPM1 mutation positive and two patients (#33 and #43) are NPM1 mutation negative. (C) The melt curve of FLT3 exon 14 and (D) The difference plot of FLT3 exon 14 - Six patient samples are shown in comparison to five normal controls. Three patients (#6, #33 and #43) are FLT3-ITD positive and three patients (#12, #14 and #48) are FLT3-ITD negative. (E) The melt curve of FLT3 exon 14 and (F) The difference plot of FLT3 exon 14 - Eight patient samples are shown in comparison to five normal controls. One patient (#19) is positive for FLT3 Y572C and seven patients (#4, #5, #10, #24, #25, #26 and #30) are FLT3 mutation negative. All samples are shown in duplicate.